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rox reference dye  (tiangen biotech co)


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    Structured Review

    tiangen biotech co rox reference dye
    Rox Reference Dye, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 99/100, based on 362 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rox+reference+dye/Reverse+Primer/pm41365136-82-16-19
    Average 99 stars, based on 362 article reviews
    rox reference dye - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Critical Analysis of Multi-Omic Data from a Strain of Plutella xylostella Resistant to Bacillus thuringiensis Cry1Ac Toxin.
    Article Snippet: Rapid evolution of resistance in crop pests to Bacillus thuringiensis (Bt) products threatens their widespread use, especially as pests appear to develop resistance through a range of different physiological adaptations.. With such a diverse range of mechanisms reported, researchers have resorted to multi-omic approaches to understand the molecular basis of resistance.. Such approaches generate a lot of data making it difficult to establish where causal links between physiological changes and resistance exist.

    Article Title: Delaying wheat sowing date and increasing sowing rate promotes lignin synthesis and reduces lodging
    Article Snippet: First-strand cDNA was synthesized from 1 lg of total RNA using the FastKing RT Kit (TIANGEN, China). f The quantitative PCR (qPCR) assay was conducted using the 7500 Real-Time PCR instrument (ABI, UK). .. The reaction mixture comprised 2 lL of diluted cDNA as template, 10 lL o 2 FastReal qPCR PreMix (TIANGEN, China), 0.6 lL each of 10 lmol L 1 forward and reverse primers (300 nmol L 1 final concentration each), 1 lL of ROX Reference Dye (50 ; TIANGEN, China), and 5.8 lL of nuclease-free water, making a total volume of 20 lL. ..

    Article Title: Environmental microorganisms as heterogeneous sources and shapers of the fermentation microbiome in Zhejiang rosy vinegar.
    Article Snippet: Zhejiang rosy vinegar (ZRV) fermentation is a complex, spontaneous open-system process, where microbial communities are key to its unique characteristics.. However, the precise influence of the brewing workshop environment on these dynamic microbial communities remains underexplored, hindering consistent product quality.. Here, we characterized the environmental and fermentation microbial communities across three factories using high-throughput sequencing (16S rRNA and ITS), qPCR, and advanced ecological network analyses.

    Article Title: Comparative transcriptome and miRNA analysis of skin pigmentation during embryonic development of Chinese soft-shelled turtle ( Pelodiscus sinensis )
    Article Snippet: Total small RNAs were extracted from skins using the miRcute miRNA Isolation Kit (Tiangen, Beijing, China). .. Poly (A) tail addition and the reverse transcription of 2 μg RNA were performed using the miRcute miRNA First- Strand cDNA synthesis Kit (Tiangen, Beijing, China). qRT ‒ PCR was then performed using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China). qPCR was conducted in 10 μL of PCR solution containing 1 μL cDNA, 5 μL miRcute miRNA premix (2X), 1 μL of 50 × ROX Reference Dye, 0.2 μL forward primer, 0.2 μL reverse primer, and 2.6 μL ddH2O according to the following program: 95 °C for 15 s for denaturation, followed by 40 cycles of 95 °C for 20 s, 60 °C for 15 s and 72 °C for 15 s. The U6 was used as the internal control for the normalization of expression levels. ..

    Quantitative RT-PCR:

    Article Title: Neonatal heart tissue-derived EVs alleviate adult ischemic cardiac injury via regulating the function of macrophages and cardiac regeneration in murine models.
    Article Snippet: Previous studies confirmed the regenerative capacity of the mammalian neonatal heart.. We recently found that adult heart tissue-derived EVs can protect the heart from myocardial ischemia–reperfusion (I/R).. However, the role of EVs from neonatal heart tissue in cardiac healing post-ischemia remains unclear.

    Article Title: The Physiological Responses of Maize Seedlings with Different Amylose Content to Drought Stress
    Article Snippet: .. The SuperReal PreMix Color (SYBR Green) kit (Tiangen, Beijing, China) was used for qRT-PCR with a total volume of 20 μL, including 2 × SuperReal Color Premix (10 μL), 50 × ROX Reference Dye (0.4 μL), forward primer (0.6 μL), reverse primer (0.6 μL), cDNA (2 μL), and RNase-free ddH2O (6.4 μL). ..

    Article Title: The effects of drought treatments on biosynthesis and structure of maize starches with different amylose content.
    Article Snippet: We investigated the effects of drought stress (DS) on maize varieties with different amylose content (AC).. In starches with AC of 33 %, DS increased the contents of amylopectin (AP) chains with a degree of polymerization (DP) > 36 and decreased the AP chains with DP ≤ 36, while the AC was unchanged.. DS decreased the crystallinity, the thickness of both amorphous and crystalline lamellae, and average granular size.

    Concentration Assay:

    Article Title: Delaying wheat sowing date and increasing sowing rate promotes lignin synthesis and reduces lodging
    Article Snippet: First-strand cDNA was synthesized from 1 lg of total RNA using the FastKing RT Kit (TIANGEN, China). f The quantitative PCR (qPCR) assay was conducted using the 7500 Real-Time PCR instrument (ABI, UK). .. The reaction mixture comprised 2 lL of diluted cDNA as template, 10 lL o 2 FastReal qPCR PreMix (TIANGEN, China), 0.6 lL each of 10 lmol L 1 forward and reverse primers (300 nmol L 1 final concentration each), 1 lL of ROX Reference Dye (50 ; TIANGEN, China), and 5.8 lL of nuclease-free water, making a total volume of 20 lL. ..

    SYBR Green Assay:

    Article Title: The Physiological Responses of Maize Seedlings with Different Amylose Content to Drought Stress
    Article Snippet: .. The SuperReal PreMix Color (SYBR Green) kit (Tiangen, Beijing, China) was used for qRT-PCR with a total volume of 20 μL, including 2 × SuperReal Color Premix (10 μL), 50 × ROX Reference Dye (0.4 μL), forward primer (0.6 μL), reverse primer (0.6 μL), cDNA (2 μL), and RNase-free ddH2O (6.4 μL). ..

    Article Title: The effects of drought treatments on biosynthesis and structure of maize starches with different amylose content.
    Article Snippet: We investigated the effects of drought stress (DS) on maize varieties with different amylose content (AC).. In starches with AC of 33 %, DS increased the contents of amylopectin (AP) chains with a degree of polymerization (DP) > 36 and decreased the AP chains with DP ≤ 36, while the AC was unchanged.. DS decreased the crystallinity, the thickness of both amorphous and crystalline lamellae, and average granular size.

    Article Title: Comparative transcriptome and miRNA analysis of skin pigmentation during embryonic development of Chinese soft-shelled turtle ( Pelodiscus sinensis )
    Article Snippet: Total small RNAs were extracted from skins using the miRcute miRNA Isolation Kit (Tiangen, Beijing, China). .. Poly (A) tail addition and the reverse transcription of 2 μg RNA were performed using the miRcute miRNA First- Strand cDNA synthesis Kit (Tiangen, Beijing, China). qRT ‒ PCR was then performed using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China). qPCR was conducted in 10 μL of PCR solution containing 1 μL cDNA, 5 μL miRcute miRNA premix (2X), 1 μL of 50 × ROX Reference Dye, 0.2 μL forward primer, 0.2 μL reverse primer, and 2.6 μL ddH2O according to the following program: 95 °C for 15 s for denaturation, followed by 40 cycles of 95 °C for 20 s, 60 °C for 15 s and 72 °C for 15 s. The U6 was used as the internal control for the normalization of expression levels. ..

    Sterility:

    Article Title: Environmental microorganisms as heterogeneous sources and shapers of the fermentation microbiome in Zhejiang rosy vinegar.
    Article Snippet: Zhejiang rosy vinegar (ZRV) fermentation is a complex, spontaneous open-system process, where microbial communities are key to its unique characteristics.. However, the precise influence of the brewing workshop environment on these dynamic microbial communities remains underexplored, hindering consistent product quality.. Here, we characterized the environmental and fermentation microbial communities across three factories using high-throughput sequencing (16S rRNA and ITS), qPCR, and advanced ecological network analyses.

    Reverse Transcription:

    Article Title: Comparative transcriptome and miRNA analysis of skin pigmentation during embryonic development of Chinese soft-shelled turtle ( Pelodiscus sinensis )
    Article Snippet: Total small RNAs were extracted from skins using the miRcute miRNA Isolation Kit (Tiangen, Beijing, China). .. Poly (A) tail addition and the reverse transcription of 2 μg RNA were performed using the miRcute miRNA First- Strand cDNA synthesis Kit (Tiangen, Beijing, China). qRT ‒ PCR was then performed using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China). qPCR was conducted in 10 μL of PCR solution containing 1 μL cDNA, 5 μL miRcute miRNA premix (2X), 1 μL of 50 × ROX Reference Dye, 0.2 μL forward primer, 0.2 μL reverse primer, and 2.6 μL ddH2O according to the following program: 95 °C for 15 s for denaturation, followed by 40 cycles of 95 °C for 20 s, 60 °C for 15 s and 72 °C for 15 s. The U6 was used as the internal control for the normalization of expression levels. ..

    cDNA Synthesis:

    Article Title: Comparative transcriptome and miRNA analysis of skin pigmentation during embryonic development of Chinese soft-shelled turtle ( Pelodiscus sinensis )
    Article Snippet: Total small RNAs were extracted from skins using the miRcute miRNA Isolation Kit (Tiangen, Beijing, China). .. Poly (A) tail addition and the reverse transcription of 2 μg RNA were performed using the miRcute miRNA First- Strand cDNA synthesis Kit (Tiangen, Beijing, China). qRT ‒ PCR was then performed using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China). qPCR was conducted in 10 μL of PCR solution containing 1 μL cDNA, 5 μL miRcute miRNA premix (2X), 1 μL of 50 × ROX Reference Dye, 0.2 μL forward primer, 0.2 μL reverse primer, and 2.6 μL ddH2O according to the following program: 95 °C for 15 s for denaturation, followed by 40 cycles of 95 °C for 20 s, 60 °C for 15 s and 72 °C for 15 s. The U6 was used as the internal control for the normalization of expression levels. ..

    Polymerase Chain Reaction:

    Article Title: Comparative transcriptome and miRNA analysis of skin pigmentation during embryonic development of Chinese soft-shelled turtle ( Pelodiscus sinensis )
    Article Snippet: Total small RNAs were extracted from skins using the miRcute miRNA Isolation Kit (Tiangen, Beijing, China). .. Poly (A) tail addition and the reverse transcription of 2 μg RNA were performed using the miRcute miRNA First- Strand cDNA synthesis Kit (Tiangen, Beijing, China). qRT ‒ PCR was then performed using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China). qPCR was conducted in 10 μL of PCR solution containing 1 μL cDNA, 5 μL miRcute miRNA premix (2X), 1 μL of 50 × ROX Reference Dye, 0.2 μL forward primer, 0.2 μL reverse primer, and 2.6 μL ddH2O according to the following program: 95 °C for 15 s for denaturation, followed by 40 cycles of 95 °C for 20 s, 60 °C for 15 s and 72 °C for 15 s. The U6 was used as the internal control for the normalization of expression levels. ..

    Control:

    Article Title: Comparative transcriptome and miRNA analysis of skin pigmentation during embryonic development of Chinese soft-shelled turtle ( Pelodiscus sinensis )
    Article Snippet: Total small RNAs were extracted from skins using the miRcute miRNA Isolation Kit (Tiangen, Beijing, China). .. Poly (A) tail addition and the reverse transcription of 2 μg RNA were performed using the miRcute miRNA First- Strand cDNA synthesis Kit (Tiangen, Beijing, China). qRT ‒ PCR was then performed using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China). qPCR was conducted in 10 μL of PCR solution containing 1 μL cDNA, 5 μL miRcute miRNA premix (2X), 1 μL of 50 × ROX Reference Dye, 0.2 μL forward primer, 0.2 μL reverse primer, and 2.6 μL ddH2O according to the following program: 95 °C for 15 s for denaturation, followed by 40 cycles of 95 °C for 20 s, 60 °C for 15 s and 72 °C for 15 s. The U6 was used as the internal control for the normalization of expression levels. ..

    Expressing:

    Article Title: Comparative transcriptome and miRNA analysis of skin pigmentation during embryonic development of Chinese soft-shelled turtle ( Pelodiscus sinensis )
    Article Snippet: Total small RNAs were extracted from skins using the miRcute miRNA Isolation Kit (Tiangen, Beijing, China). .. Poly (A) tail addition and the reverse transcription of 2 μg RNA were performed using the miRcute miRNA First- Strand cDNA synthesis Kit (Tiangen, Beijing, China). qRT ‒ PCR was then performed using the miRcute Plus miRNA qPCR Kit (SYBR Green) (Tiangen, Beijing, China). qPCR was conducted in 10 μL of PCR solution containing 1 μL cDNA, 5 μL miRcute miRNA premix (2X), 1 μL of 50 × ROX Reference Dye, 0.2 μL forward primer, 0.2 μL reverse primer, and 2.6 μL ddH2O according to the following program: 95 °C for 15 s for denaturation, followed by 40 cycles of 95 °C for 20 s, 60 °C for 15 s and 72 °C for 15 s. The U6 was used as the internal control for the normalization of expression levels. ..



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